Off-target detection
Defining genome-wide CRISPR–Cas genome-editing nuclease activity with GUIDE-seq
6 related method titles
Malinin et al., Nature Protocols (2021)
Cell & Gene Therapy (CGT) Protocol References
Find cell and gene therapy publication references for gene-editing off-target detection, LNP delivery, cell culture and cell analysis. Follow original-source links.
Gene-editing Off-target Detection ReferencesBrowse GUIDE-seq, PEM-seq and Detect-seq literature by method family. Find publication citations, original-source links and related experiment titles.Delivery Protocols for Cell & Gene Therapy
Find targeted LNP and SORT LNP delivery publication references, source citations and related method titles for cell and gene therapy research.Cell Culture & Transfection Protocols
Find cell culture and transfection publication references, including HEK293-related method titles, source citations and links to original research.Cell Analysis Protocols
Find cell analysis and cell-death flow cytometry publication references, source links and related experiment titles.
9 article collections available.
Article references
Each card links to publication information and related method titles. Detailed steps and executable downloads have been withdrawn.
Popular tags by experiment count
9 collections · 47 experiments
Off-target detection
Detect-seq, a chemical labeling and biotin pull-down approach for the unbiased and genome-wide off-target evaluation of programmable cytosine base editors
4 related method titles
Lei et al., Nature Protocols (2023)
Cell culture & transfection
DNA targeting specificity of RNA-guided Cas9 nucleases
1 related method titles
Hsu et al., Nature Biotechnology (2013)
View experiment titles
Cell analysis
Monitoring the progression of cell death and the disassembly of dying cells by flow cytometry
5 related method titles
Jiang et al., Nature Protocols (2016)
Off-target detection
PEM-seq comprehensively quantifies DNA repair outcomes during gene-editing and DSB repair
10 related method titles
Liu et al., STAR Protocols (2022)
View experiment titles
- PEM-seq cell lysis & DNA extraction Day2
- PEM-seq cell preparation Day2
- PEM-seq cell preparation Day4
- PEM-seq DNA fragmentation
- PEM-seq molecular preparation Day1
- PEM-seq molecular preparation Day2
- PEM-seq molecular preparation Day3
- PEM-seq molecular preparation Day4
- PEM-seq molecular preparation Day5
- PEM-seq sequencing & analysis Day2
Delivery
Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery
15 related method titles
Wang et al., Nature Protocols (2023)
View experiment titles
- SORT LNP 4A3-SC8 lipid stock and mix preparation
- SORT LNP 4A3-SC8 purification and characterization
- SORT LNP 4A3-SC8 synthesis Day 1
- SORT LNP 4A3-SC8 synthesis Day 2
- SORT LNP AEMA synthesis Day 1
- SORT LNP AEMA synthesis Day 2
- SORT LNP in vitro delivery evaluation
- SORT LNP in vivo delivery evaluation
- SORT LNP MC3 lipid stock and mix preparation
- SORT LNP microfluidic mixing formulation
- SORT LNP particle size characterization
- SORT LNP pipette mixing formulation
- SORT LNP reagent and buffer preparation
- SORT LNP RNA encapsulation assay
- SORT LNP vortex mixing formulation
Delivery
Preparation of targeted lipid nanoparticles for precision nucleic acid delivery
4 related method titles
Geisler et al., Nature Protocols (2026)
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Cell culture and transfection methods are included above as supporting CGT workflows.
Sources and use
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