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Gene-editing Off-target Detection References

Browse GUIDE-seq, PEM-seq and Detect-seq literature by method family. Find publication citations, original-source links and related experiment titles.

GUIDE-seq Protocols
Find GUIDE-seq publication references and related method titles for cell preparation, library preparation and sequencing. Open the original papers for methods.PEM-seq Protocols
Find PEM-seq publication references and related titles for DNA extraction, library preparation and sequencing. Follow links to the original publications.Detect-seq Protocols
Find Detect-seq publication citations and related method titles for library preparation, quality control and sequencing, with original-source links.

5 article collections available.

Article references

Each card links to publication information and related method titles. Detailed steps and executable downloads have been withdrawn.

5 collections · 22 experiments

Off-target detection

Detect-seq, a chemical labeling and biotin pull-down approach for the unbiased and genome-wide off-target evaluation of programmable cytosine base editors

4 related method titles

Lei et al., Nature Protocols (2023)

Base-editor off-target detectionDetect-seqDNA fragmentationfirst PCR amplificationGene editingIn vitroLibrary preparationNGSOff-target detectionQC & sequencingSample preparationsecond PCR amplificationSequencing & analysisstreptavidin pull-downY-adapter ligation and strand denaturation

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Off-target detection

PEM-seq comprehensively quantifies DNA repair outcomes during gene-editing and DSB repair

10 related method titles

Liu et al., STAR Protocols (2022)

Cell preparationDNA extractionDNA fragmentationMolecular preparationOff-target detectionPEM-seqSequencing & analysis

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Sources and use

Individual pages list available source citations. Consult the cited publication for experimental methods and context.