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Detect-seq Protocols

Find Detect-seq publication citations and related method titles for library preparation, quality control and sequencing, with original-source links.

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1 collections · 4 experiments

Off-target detection

Detect-seq, a chemical labeling and biotin pull-down approach for the unbiased and genome-wide off-target evaluation of programmable cytosine base editors

4 related method titles

Lei et al., Nature Protocols (2023)

Base-editor off-target detectionDetect-seqDNA fragmentationfirst PCR amplificationGene editingIn vitroLibrary preparationNGSOff-target detectionQC & sequencingSample preparationsecond PCR amplificationSequencing & analysisstreptavidin pull-downY-adapter ligation and strand denaturation

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